Cunliffe Michael, Salter Matthew, Mann Paul J, Whiteley Andrew S, Upstill-Goddard Robert C, Murrell J Colin
Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry, UK.
FEMS Microbiol Lett. 2009 Oct;299(2):248-54. doi: 10.1111/j.1574-6968.2009.01751.x. Epub 2009 Aug 6.
The sea surface microlayer is the interfacial boundary layer between the marine environment and the troposphere. Surface microlayer samples were collected during a fjord mesocosm experiment to study microbial assemblage dynamics within the surface microlayer during a phytoplankton bloom. Transparent exopolymer particles were significantly enriched in the microlayer samples, supporting the concept of a gelatinous surface film. Dissolved organic carbon and bacterial cell numbers (determined by flow cytometry) were weakly enriched in the microlayer samples. However, the numbers of Bacteria 16S rRNA genes (determined by quantitative real-time PCR) were more variable, probably due to variable numbers of bacterial cells attached to particles. The enrichment of transparent exopolymer particles in the microlayer and the subsequent production of a gelatinous biofilm have implications on air-sea gas transfer and the partitioning of organic carbon in surface waters.
海洋表面微层是海洋环境与对流层之间的界面边界层。在峡湾中尺度生态系统实验期间采集了表面微层样本，以研究浮游植物大量繁殖期间表面微层内的微生物群落动态。透明胞外聚合物颗粒在微层样本中显著富集，支持了凝胶状表面膜的概念。溶解有机碳和细菌细胞数量（通过流式细胞术测定）在微层样本中略有富集。然而，细菌16S rRNA基因数量（通过定量实时PCR测定）变化更大，可能是由于附着在颗粒上的细菌细胞数量不同。微层中透明胞外聚合物颗粒的富集以及随后凝胶状生物膜的形成对海气气体交换和表层水体中有机碳的分配具有影响。
