Department of Microbiology, University of Washington, Seattle, WA 98195-1750, United States.
Syst Appl Microbiol. 2012 May;35(3):198-203. doi: 10.1016/j.syapm.2011.10.006. Epub 2012 Mar 9.
Saanich Inlet (British Columbia, Canada) is a seasonally anoxic fjord characterized by high rates of both methane production and consumption. In this study, the diversity of microbial populations residing in intermediate waters, characterized by having a high methane content, was assessed using CH(4)-microcosm experiments coupled with PCR surveys of phylogenetic (16S rRNA gene) and functional gene markers (pmoA and fhcD genes). The experiments revealed that bacteria represented by sequences affiliated with Methylomicrobium within the Methylococcales, Methylophaga and Cycloclasticus within the Thiotrichales, and uncultured Planctomycetes were enriched in response to CH(4) addition.
加拿大不列颠哥伦比亚省的萨尼奇湾是一个季节性缺氧的峡湾，其甲烷的产生和消耗速率都很高。在这项研究中，利用 CH(4)-微宇宙实验和对系统发育（16S rRNA 基因）和功能基因标记（pmoA 和 fhcD 基因）的 PCR 调查，评估了栖息在中间水域（具有高甲烷含量）的微生物种群的多样性。实验表明，在甲烷添加的刺激下，甲基球菌目内的甲基微菌属、硫发菌目内的噬甲基菌属和噬甲基球菌属以及未培养的浮霉菌门的细菌序列得到了富集。
