Sir William Dunn School of Pathology, South Parks Road, University of Oxford, OX1 3RE, UK.
Cell Commun Signal. 2009 Sep 4;7:21. doi: 10.1186/1478-811X-7-21.
Considerable controversy arose over the concept that cholesterol/sphingolipid-rich rafts in the T cell plasma membrane serve as a platform for TCR signalling reactions. This controversy was founded on the initial definition of rafts as detergent resistant membranes which later turned out to misrepresent many features of cell membrane organisation under physiological conditions. Raft-organisation was subsequently studied using a number of detergent-free experimental approaches. The results led to a refined perception of membrane rafts which resolves the controversies. Here we review new biophysical and biochemical data which provide an updated picture of the highly dynamic nanometer-sized cholesterol/sphingolipid-rich raft domains stabilised by protein-networks to form TCR signalling platforms in the T cell plasma membrane.
关于胆固醇/神经鞘脂类富含的筏在 T 细胞质膜中充当 TCR 信号反应平台的概念，引起了相当大的争议。这种争议是基于最初将筏定义为去污剂抗性膜，而后来发现这种定义不能代表生理条件下细胞膜组织的许多特征。随后，使用多种非去污剂实验方法研究了筏组织。这些结果导致了对膜筏的精细认识，解决了争议。在这里，我们回顾了新的生物物理和生化数据，这些数据提供了一个更新的图片，即高度动态的纳米级胆固醇/神经鞘脂类富含的筏域由蛋白质网络稳定，在 T 细胞质膜中形成 TCR 信号平台。
