Lijnen H R, Van Hoef B, Rodriguez J A, Paramo J A
Center for Molecular and Vascular Biology, KU Leuven, B-3000 Leuven, Belgium.
Biochem Biophys Res Commun. 2009 Nov 13;389(2):378-81. doi: 10.1016/j.bbrc.2009.08.170. Epub 2009 Sep 2.
Adipose tissue development is associated with angiogenesis, adipogenesis and extracellular matrix degradation. The class of matrix metalloproteinases contributes to these processes, but little information is available on the role of individual proteinases. We report that stromelysin-2 (MMP-10) deficiency has no significant effect on total body weight or on subcutaneous (SC) or gonadal (GON) adipose tissue mass of mice kept on a high fat diet for 15 weeks. The adipocyte size and density in SC and GON adipose tissues were also comparable in MMP-10 deficient and wild-type control mice. Similarly, blood vessel size and density in obese SC and GON adipose tissues was not affected by MMP-10 deficiency. Metabolic parameters and blood cell composition were similar for both genotypes. Stromelysin-1 (MMP-3) expression was significantly reduced in adipose tissues of the deficient mice as compared to the wild-type controls. These data indicate that MMP-10 does not significantly contribute to adipose tissue development and associated angiogenesis in a mouse model of nutritionally induced obesity.
脂肪组织的发育与血管生成、脂肪生成和细胞外基质降解相关。基质金属蛋白酶家族参与了这些过程，但关于单个蛋白酶的作用，我们所知甚少。我们报道，基质溶素-2（MMP-10）缺乏对高脂饮食喂养15周的小鼠的总体重、皮下（SC）或性腺（GON）脂肪组织量没有显著影响。MMP-10缺陷小鼠和野生型对照小鼠的SC和GON脂肪组织中的脂肪细胞大小和密度也相当。同样，肥胖的SC和GON脂肪组织中的血管大小和密度不受MMP-10缺乏的影响。两种基因型的代谢参数和血细胞组成相似。与野生型对照相比，缺陷小鼠脂肪组织中基质溶素-1（MMP-3）的表达显著降低。这些数据表明，在营养诱导肥胖的小鼠模型中，MMP-10对脂肪组织发育和相关血管生成没有显著贡献。
