AngioLab, Inc, Daejeon, Korea.
Int J Obes (Lond). 2010 May;34(5):820-30. doi: 10.1038/ijo.2010.13. Epub 2010 Feb 23.
The growth and development of adipose tissue are thought to be associated with angiogenesis and extracellular matrix remodeling. As the composition of the herbal extract called Ob-X has been shown to have both anti-angiogenic and matrix metalloproteinase (MMP)-inhibiting activities, we hypothesized that growth of adipose tissue can be regulated by Ob-X.
The effects of Ob-X on angiogenesis and extracellular matrix remodeling were measured using in vitro and ex vivo assays. The effects of Ob-X on adipose tissue growth were investigated in nutritionally obese mice.
Ob-X inhibited angiogenesis in a dose-dependent manner in the human umbilical vein endothelial cell tube formation assay in vitro and the rat aortic ring assay ex vivo. Ob-X also suppressed MMP activity in vitro. Administration of Ob-X to high fat diet-induced obese mice produced significant reductions in body weight gain and adipose tissue mass compared with control. The mass of both visceral (VSC) and subcutaneous (SC) fat was reduced in Ob-X-treated mice. The size of adipocytes in VSC and SC adipose tissues was also significantly reduced in Ob-X-treated mice. Ob-X treatment decreased the blood vessel density and MMP activity in VSC adipose tissues of nutritionally obese mice. Ob-X reduced mRNA levels of angiogenic factors (VEGF-A and FGF-2) and MMPs (MMP-2 and MMP-9), whereas it increased mRNA levels of angiogenic inhibitors (TSP-1, TIMP-1 and TIMP-2) in SC and VSC adipose tissues of nutritionally obese mice.
Ob-X, which has anti-angiogenic and MMP-inhibitory activities, reduces adipose tissue mass in nutritionally induced obese mice, providing evidence that adipose tissue growth and development may be prevented by inhibiting angiogenesis. In addition, these data suggest that regulation of adipose tissue growth by inhibiting angiogenesis may alter the expression of genes involved in angiogenesis and the MMP system.
脂肪组织的生长和发育被认为与血管生成和细胞外基质重塑有关。由于名为 Ob-X 的草药提取物的成分已被证明具有抗血管生成和基质金属蛋白酶（MMP）抑制活性，我们假设 Ob-X 可以调节脂肪组织的生长。
使用体外和离体测定法测量 Ob-X 对血管生成和细胞外基质重塑的影响。在营养肥胖小鼠中研究 Ob-X 对脂肪组织生长的影响。
Ob-X 以剂量依赖的方式抑制体外人脐静脉内皮细胞管形成试验和大鼠主动脉环试验中的血管生成。Ob-X 还抑制了体外的 MMP 活性。与对照组相比，给予 Ob-X 可使高脂肪饮食诱导的肥胖小鼠体重增加和脂肪组织质量显著减少。Ob-X 处理的小鼠内脏（VSC）和皮下（SC）脂肪的质量均降低。Ob-X 处理的小鼠 VSC 和 SC 脂肪组织中的脂肪细胞大小也明显减小。Ob-X 处理降低了营养性肥胖小鼠 VSC 脂肪组织中的血管密度和 MMP 活性。Ob-X 降低了 SC 和 VSC 脂肪组织中血管生成因子（VEGF-A 和 FGF-2）和 MMP（MMP-2 和 MMP-9）的 mRNA 水平，而增加了营养性肥胖小鼠 SC 和 VSC 脂肪组织中血管生成抑制剂（TSP-1、TIMP-1 和 TIMP-2）的 mRNA 水平。
具有抗血管生成和 MMP 抑制活性的 Ob-X 可减少营养诱导肥胖小鼠的脂肪组织质量，这表明抑制血管生成可能会阻止脂肪组织的生长和发育。此外，这些数据表明，通过抑制血管生成来调节脂肪组织的生长可能会改变参与血管生成和 MMP 系统的基因的表达。
