College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
Biosens Bioelectron. 2009 Dec 15;25(4):708-14. doi: 10.1016/j.bios.2009.08.013. Epub 2009 Aug 15.
Copper oxide nanofibers (CuO-NFs) prepared by electrospinning and subsequent thermal treatment processes were demonstrated for the first time for glucose non-enzymatic determination. The structures and morphologies of CuO-NFs were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-ray diffraction spectrum (XRD). Different dispersants were utilized for the suspension preparation and effects of ultrasonic time on the films electrode fabrication were investigated in detail. The assay performances to glucose were evaluated by cyclic voltammetry (CV) and chronoamperometry (I-t). Results revealed a high sensitivity (431.3 microAmM(-1)cm(-2)), fast response (about 1s), long-term stability and excellent resistance towards electrode fouling in the glucose determination at +0.40V. The improved performances of CuO-NFs films electrode for electro-oxidation glucose were ascribed to the high surface-to-volume ratio, complex pore structure, extremely long length of the as-prepared CuO-NFs, and the excellent three-dimensional network structure after immobilization. Results in this study suggest that electrospun CuO-NFs is a promising 1-D nanomaterial for further design and microfabrication of bioelectrochemical nanodevices for glucose determination.
氧化铜纳米纤维（CuO-NFs）通过静电纺丝和后续的热处理过程制备，首次被证明可用于葡萄糖的非酶检测。CuO-NFs 的结构和形态通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）和 X 射线衍射光谱（XRD）进行了表征。利用不同的分散剂来制备悬浮液，并详细研究了超声时间对薄膜电极制备的影响。通过循环伏安法（CV）和计时电流法（I-t）评估了对葡萄糖的测定性能。结果表明，在+0.40V 下测定葡萄糖时，该传感器具有高灵敏度（431.3 μA mM-1 cm-2）、快速响应（约 1s）、长期稳定性和对电极污染的优异抗性。CuO-NFs 薄膜电极对电氧化葡萄糖性能的提高归因于高的比表面积-体积比、复杂的孔结构、所制备的 CuO-NFs 的超长长度以及固定化后的优异三维网络结构。本研究结果表明，电纺 CuO-NFs 是一种很有前途的一维纳米材料，可进一步设计和微制造用于葡萄糖检测的生物电化学纳米器件。
