Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Res Microbiol. 2009 Oct;160(8):560-6. doi: 10.1016/j.resmic.2009.08.010. Epub 2009 Sep 4.
An extremely thermophilic alkane-degrading bacterium, strain MH-1, was isolated from the deep subterranean petroleum reservoir in Shengli oil field, PR China. Based on its physiological characteristics and analysis of its 16S rRNA gene sequence, strain MH-1 was identified as Geobacillus stearothermophilus. Strain MH-1 was able to grow at temperatures ranging from 50 to 72 degrees C and effectively degraded hexadecane as the sole carbon source at 70 degrees C. Strain MH-1 degraded alkanes with different length chains (C(12)-C(31)) in crude oil, but it preferentially degraded middle chain-alkanes. The alkane hydroxylase system of G. stearothermophilus MH-1 was characterized. It contained at least three alkane monooxygenase gene homologs (alkB-geo1, alkB-geo4, and alkB-geo6). The AlkB-geo6 cluster, which had a high sequence similarity to the alkB2 cluster of Rhodococcus strains NRRL B-16531 and Q15, contained alkB-geo6, two rubredoxins and a putative transcriptional regulatory protein.
从中国胜利油田深部地下油藏中分离到一株嗜热烷烃降解菌，命名为 MH-1 株。根据其生理特性和 16S rRNA 基因序列分析，鉴定该菌株为嗜热脂肪地芽孢杆菌。MH-1 株能够在 50-72°C 的温度范围内生长，并能以十六烷作为唯一碳源在 70°C 时有效地降解。MH-1 株能降解原油中不同链长的烷烃（C(12)-C(31))，但优先降解中链烷烃。对嗜热脂肪地芽孢杆菌 MH-1 的烷烃羟化酶系统进行了表征。它至少含有三个烷烃单加氧酶基因同源物（alkB-geo1、alkB-geo4 和 alkB-geo6）。AlkB-geo6 簇与 Rhodococcus 菌株 NRRL B-16531 和 Q15 的 alkB2 簇具有很高的序列相似性，包含 alkB-geo6、两个 rubredoxins 和一个假定的转录调节蛋白。
