Yu Tianfei, Liu Xiaodong, Ai Jiamin, Wang Jiamin, Guo Yidan, Liu Xinhui, He Xiaolong, Deng Zhenshan, Jiang Yingying
College of Life Sciences, Yan'an University, Yan'an, China.
Front Microbiol. 2022 Nov 4;13:1044448. doi: 10.3389/fmicb.2022.1044448. eCollection 2022.
Microbial community succession during the enrichment of crude-oil-degrading bacteria was analyzed using Illumina high-throughput sequencing to guide bacterial isolation and construction of a bacterial consortium. Community change occurred in 6 days; the most abundant phylum changed from Proteobacteria to Actinobacteria; the most abundant genera were and . Two crude oil-degrading strains, sp. OS62-1 and sp. OS33, and one weak-crude-oil-degrading strain, sp. P35, were isolated. A consortium comprising sp. OS62-1 and sp. P35 showed the highest crude-oil-degrading efficiency, reaching 85.72 ± 3.21% within 7 days, over a wide pH range (5-11) and salinity (0-80 g·L). Consumption of saturated hydrocarbons, aromatic hydrocarbons, and resins was greater by the consortium than by a single strain, as was degradation of short-chain-alkanes (C-C) according to gas-chromatography. The bacterial consortium provides technical support for bioremediation of crude oil pollution.
利用Illumina高通量测序技术分析原油降解菌富集过程中的微生物群落演替，以指导细菌分离和构建细菌联合体。群落变化在6天内发生；最丰富的门从变形菌门变为放线菌门；最丰富的属是 和 。分离出两株原油降解菌，即 菌OS62-1和 菌OS33，以及一株弱原油降解菌， 菌P35。由 菌OS62-1和 菌P35组成的联合体显示出最高的原油降解效率，在7天内达到85.72±3.21%，在较宽的pH范围（5-11）和盐度（0-80 g·L）内均如此。根据气相色谱分析，该联合体对饱和烃、芳烃和树脂的消耗大于单一菌株，对短链烷烃（C-C）的降解也是如此。该细菌联合体为原油污染的生物修复提供了技术支持。
