Komiyama Nobuko, Hiemori Miki, Kimoto Masumi, Suzuki Makiko, Yamashita Hiromi, Takahashi Yoshitaka, Tsuji Hideaki
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
Biosci Biotechnol Biochem. 2009 Sep;73(9):2113-6. doi: 10.1271/bbb.90224. Epub 2009 Sep 7.
We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence (154)VPWVVVDGKPL(164) of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.
我们制备了一种单克隆抗体（mAb）作为检测小麦主要过敏原Tri a Bd 27K的探针。该单克隆抗体识别从小麦粉中纯化的过敏原，并且确定该过敏原上与单克隆抗体结合的表位为Tri a Bd 27K的氨基酸序列（154）VPWVVVDGKPL（164）。在该表位的氨基酸残基中，发现与单克隆抗体结合的氨基酸残基为W156、D160、G161和P163。
