花生过敏原Ara h1与人单克隆IgM抗体克隆#86的表位分析。

Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody clone #86.

作者信息

Shinmoto Hiroshi, Takeda Mio, Matsuo Yuji, Naganawa Yasunori, Tomita Shinichi, Takano-Ishikawa Yuko

机构信息

Tamagawa University, Machida, Tokyo, Japan.

出版信息

Hum Antibodies. 2010;19(4):101-5. doi: 10.3233/HAB-2010-0233.

DOI:10.3233/HAB-2010-0233

PMID:21178281

Abstract

A human-mouse hybridoma clone #86 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was newly established. To detect an antibody-binding sequence (epitope) on Ara h1, the monoclonal antibody #86 was reacted with multi-pin apparatus with a series of peptides synthesized from the amino acid sequence of Ara h1. The antibody #86 was found to bind to a peptide with amino acid sequence of 481EEEEDEDEEEEGSNREVRRY500. Further analysis with shorter pin-peptides with ten amino acid-long showed that the peptides reacted with the antibody #86 contained a sequence of 485DEDEEEE491. This might be an essential linear sequence of this epitope. When the 485DED487 part of the peptide was replaced by alanine, decreased binding of antibody #86 was observed.

摘要

新建立了一株人-鼠杂交瘤克隆#86，它能分泌针对花生过敏原蛋白Ara h1的IgM类人单克隆抗体。为了检测Ara h1上的抗体结合序列（表位），将单克隆抗体#86与多针装置反应，该装置带有一系列根据Ara h1氨基酸序列合成的肽段。发现抗体#86能与氨基酸序列为481EEEEDEDEEEEGSNREVRRY500的肽段结合。用十个氨基酸长度的更短针肽进一步分析表明，与抗体#86反应的肽段包含序列485DEDEEEE491。这可能是该表位的一个基本线性序列。当肽段的485DED487部分被丙氨酸取代时，观察到抗体#86的结合减少。