体外液压诱导肾小管上皮细胞-肌成纤维细胞转分化
Hydraulic pressure inducing renal tubular epithelial-myofibroblast transdifferentiation in vitro.
作者信息
Li Fei-yan, Xie Xi-sheng, Fan Jun-ming, Li Zi, Wu Jiang, Zheng Rong
机构信息
Department of Nephrology, West China Hospital of Sichuan University, Chengdu 610041, China.
出版信息
J Zhejiang Univ Sci B. 2009 Sep;10(9):659-67. doi: 10.1631/jzus.B0920110.
OBJECTIVE
The effects of hydraulic pressure on renal tubular epithelial-myofibroblast transdifferentiation (TEMT) were investigated.
METHODS
We applied hydraulic pressure (50 cm H2O) to normal rat kidney tubular epithelial cells (NRK52E) for different durations. Furthermore, different pressure magnitudes were applied to cells. The morphology, cytoskeleton, and expression of myofibroblastic marker protein and transforming growth factor-beta1 (TGF-beta1) of NRK52E cells were examined.
RESULTS
Disorganized actin filaments and formation of curling clusters in actin were seen in the cytoplasm of pressurized cells. We verified that de novo expression of alpha-smooth muscle actin induced by pressure, which indicated TEMT, was dependent on both the magnitude and duration of pressure. TGF-beta1 expression was significantly upregulated under certain conditions, which implies that the induction of TEMT by hydraulic pressure is related with TGF-beta1.
CONCLUSION
We illustrate for the first time that hydraulic pressure can induce TEMT in a pressure magnitude- and duration-dependent manner, and that this TEMT is accompanied by TGF-beta1 secretion.
目的
研究液压对肾小管上皮细胞-肌成纤维细胞转分化（TEMT）的影响。
方法
我们对正常大鼠肾小管上皮细胞（NRK52E）施加不同时长的液压（50 cm H2O）。此外，对细胞施加不同的压力大小。检测NRK52E细胞的形态、细胞骨架以及肌成纤维细胞标志物蛋白和转化生长因子-β1（TGF-β1）的表达。
结果
在受压细胞的细胞质中可见肌动蛋白丝排列紊乱以及肌动蛋白卷曲簇的形成。我们证实压力诱导的α-平滑肌肌动蛋白的从头表达表明了TEMT，其依赖于压力大小和时长。在某些条件下TGF-β1表达显著上调，这意味着液压诱导的TEMT与TGF-β1有关。
结论
我们首次表明液压能够以压力大小和时长依赖的方式诱导TEMT，并且这种TEMT伴随着TGF-β1的分泌。
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