Sung Yun-Hee, Chang Hyun-Kyung, Kim Sung-Eun, Kim You-Mi, Seo Jin-Hee, Shin Min-Chul, Shin Mal-Soon, Yi Jae-Woo, Shin Dong-Hoon, Kim Hong, Kim Chang-Ju
Department of Physiology, College of Medicine, Kyung Hee University, Seoul.
J Med Food. 2009 Aug;12(4):788-95. doi: 10.1089/jmf.2008.1011.
Corni fructus is the fruit of Cornus officinalis Sieb. et Zucc, which is classified into the dogwood family of Cornaceae. Corni fructus has antineoplastic, antioxidative, and antidiabetic effects, but its anti-inflammatory and analgesic effects are unknown. Here, we investigated the anti-inflammatory and analgesic effects of an aqueous extract of corni fructus using murine RAW 264.7 macrophage cells. For this study, we used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, western blot analysis, prostaglandin (PG) E(2) immunoassay, and nitric oxide (NO) detection. In addition, the analgesic effect of corni fructus was assessed by the acetic acid-induced writhing response in mice. The aqueous extract of corni fructus suppressed PGE(2) synthesis and NO production by inhibiting the lipopolysaccharide-induced expression of cyclooxygenase (COX)-2 and inducible NO synthase (iNOS) in murine RAW 264.7 macrophage cells. The extract also suppressed increases in nuclear factor-kappaB (NF-kappaB) levels in the nucleus. In vivo study showed that the extract suppressed the acetic acid-induced writhing response in mice. The aqueous extract of corni fructus exerts anti-inflammatory and analgesic effects by suppressing COX-2 and iNOS expression through the down-regulation of NF-kappaB binding activity.
山茱萸是山茱萸科植物山茱萸的果实。山茱萸具有抗肿瘤、抗氧化和抗糖尿病作用，但其抗炎和镇痛作用尚不清楚。在此，我们使用小鼠RAW 264.7巨噬细胞研究了山茱萸水提取物的抗炎和镇痛作用。在本研究中，我们使用了3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法、蛋白质印迹分析、前列腺素（PG）E2免疫测定和一氧化氮（NO）检测。此外，通过小鼠醋酸诱导的扭体反应评估山茱萸的镇痛作用。山茱萸水提取物通过抑制小鼠RAW 264.7巨噬细胞中脂多糖诱导的环氧化酶（COX）-2和诱导型一氧化氮合酶（iNOS）的表达，抑制PGE2合成和NO产生。该提取物还抑制细胞核中核因子-κB（NF-κB）水平的升高。体内研究表明，该提取物可抑制小鼠醋酸诱导的扭体反应。山茱萸水提取物通过下调NF-κB结合活性来抑制COX-2和iNOS的表达，从而发挥抗炎和镇痛作用。
