Yamasaki Kanako, Hagiwara Hiromi
Department of Biomedical Engineering, Toin University of Yokohama, Yokohama 225-8502, Japan.
Toxicol Lett. 2009 Dec 15;191(2-3):211-5. doi: 10.1016/j.toxlet.2009.08.023. Epub 2009 Sep 6.
Hemochromatosis is an iron overload disorder associated with osteopenia and osteoporosis. To learn more about the effects of iron on bone cells, we examined the effects of ferric ion on the proliferation, differentiation, and mineralization of two types of cultured osteoblasts, the cell line MC3T3-E1 and rat calvarial osteoblast-like (ROB) cells. We used ferric ammonium citrate (FAC) as a donor of ferric ion, and FAC inhibited the proliferation of MC3T3-E1 cells in a dose-dependent manner. FAC (0.1-1 microg/ml) inhibited indices of osteoblast differentiation, such as the expression of type I collagen (mRNA and protein), the activity of alkaline phosphatase, and the deposition of calcium by osteoblasts. These results suggest that iron overload might give rise to osteoporosis by inhibiting osteoblast proliferation and differentiation.
血色素沉着症是一种与骨质减少和骨质疏松症相关的铁过载疾病。为了更多地了解铁对骨细胞的影响，我们研究了三价铁离子对两种培养的成骨细胞（MC3T3-E1细胞系和大鼠颅骨成骨样（ROB）细胞）的增殖、分化和矿化的影响。我们使用柠檬酸铁铵（FAC）作为三价铁离子的供体，FAC以剂量依赖的方式抑制MC3T3-E1细胞的增殖。FAC（0.1-1微克/毫升）抑制成骨细胞分化指标，如I型胶原蛋白（mRNA和蛋白质）的表达、碱性磷酸酶的活性以及成骨细胞对钙的沉积。这些结果表明，铁过载可能通过抑制成骨细胞的增殖和分化导致骨质疏松症。
