Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense, Brazil.
Comp Immunol Microbiol Infect Dis. 2010 Dec;33(6):485-9. doi: 10.1016/j.cimid.2009.08.003. Epub 2009 Sep 6.
In order to demonstrate the potential to distinguish paratuberculosis (PTB) from bovine tuberculosis infection (TB), ELISAs with M. bovis-specific MPB70 or MPB83 as capture antigens were developed and tested on two groups of cattle: Group A comprised 23 animals positive for Mycobacterium avium paratuberculosis (Map) and TB free. Group B comprised 48 animals from a Map free herd during the previous 5 years, but confirmed as tuberculous by positive results on PPD testing and M. bovis culture. Results demonstrated a significant difference (p<0.01) between reactivity of sera from these groups, encouraging the study of purified proteins to differentiate between both diseases.
为了证明区分副结核病（PTB）和牛型结核感染（TB）的潜力，我们开发了两种 ELISA 检测方法，使用 M. bovis 特异性 MPB70 或 MPB83 作为捕获抗原，分别在两组牛群上进行了测试：A 组包括 23 头 Map 阳性且无 TB 的动物。B 组来自于过去 5 年中 Map 阴性的牛群，但通过 PPD 检测和 M. bovis 培养阳性结果确认患有结核病。结果表明，两组血清的反应性存在显著差异（p<0.01），这鼓励我们对纯化蛋白进行研究以区分这两种疾病。
