Harrison D E, Stone M, Astle C M
Jackson Laboratory, Bar Harbor, Maine 04609.
J Exp Med. 1990 Aug 1;172(2):431-7. doi: 10.1084/jem.172.2.431.
Transplantation has strong deleterious effects on the primitive immunohematopoietic stem cells (PSC) from which circulating lymphocytes and erythrocytes are descended. We studied these effects over 300-400 d, testing whether PSC numbers, repopulating abilities, or both, were reduced. Equivalent PSC numbers were estimated in recipients of mixtures of genetically different cells, using the binomial model with covariance. Percentages of lymphocyte and erythrocyte types were closely correlated, as were percentages of either type sampled at intervals of several months. This suggests that the same PSC produced lymphoid and myeloid cells, and that most circulating cells were descended from the same PSC over hundreds of days. Equivalent PSC concentrations were approximately 1/10(5) fresh marrow cells, and were about twofold lower using previously transplanted marrow. However, such marrow repopulated only one-seventh to one-eighth as well as fresh marrow. Apparently, transplantation not only reduces PSC concentrations, but also reduces the repopulating ability per PSC. This may result from excessive stimuli to differentiate that overbalance the stimuli for PSC to replenish themselves.
移植对原始免疫造血干细胞（PSC）有强烈的有害影响，循环淋巴细胞和红细胞均起源于这些干细胞。我们在300 - 400天的时间里研究了这些影响，检测PSC数量、再增殖能力或两者是否降低。使用具有协方差的二项式模型，估计基因不同细胞混合物受体中的等效PSC数量。淋巴细胞和红细胞类型的百分比密切相关，以数月为间隔采样的任何一种类型的百分比也是如此。这表明相同的PSC产生淋巴细胞和髓细胞，并且在数百天内大多数循环细胞都起源于相同的PSC。等效PSC浓度约为1/10⁵新鲜骨髓细胞，使用先前移植的骨髓时约低两倍。然而，这种骨髓的再增殖能力仅为新鲜骨髓的七分之一至八分之一。显然，移植不仅降低了PSC浓度，还降低了每个PSC的再增殖能力。这可能是由于过度的分化刺激超过了PSC自我补充的刺激所致。
