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Salmonellae of serotypes gallinarum and pullorum grouped by biotyping and fimbrial-gene probing.

作者信息

Crichton P B, Old D C

机构信息

Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital.

出版信息

J Med Microbiol. 1990 Jul;32(3):145-52. doi: 10.1099/00222615-32-3-145.

DOI:10.1099/00222615-32-3-145
PMID:1973735
Abstract

When salmonellae of serotypes Gallinarum (50 isolates) and Pullorum (36 isolates), that produce non-adhesive (type-2) fimbriae, were tested for their reactions in biochemical tests, 81 (94%) were found to belong to three distinct biochemical groups, I-III. Interaction of HinfI-digested DNA of both Gallinarum and Pullorum with a probe of accessory genes of type-1 fimbriation in serotype Typhimurium gave one type of Southern hybridisation pattern that was readily distinguished from that of Typhimurium strains. With a probe of the Typhimurium fimbrial subunit gene, Pullorum isolates were separated into strongly and weakly probe-reactive groups which showed restriction fragment-length polymorphism; these latter groups corresponded to biochemical groups II and III, respectively.

摘要

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