Olsen J E, Skov M N, Christensen J P, Bisgaard M
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
J Med Microbiol. 1996 Dec;45(6):413-8. doi: 10.1099/00222615-45-6-413.
Forty-eight strains of Salmonella enterica serotype Gallinarum of biotypes Gallinarum and Pullorum were characterised by three chromosomally based typing methods. The patterns obtained were compared with those of strains of eight other serotypes of Salmonella of O serogroup D. The same PvuII and PstI IS200 patterns were commonly observed among strains of both biotypes and the three SmaI ribotypes of serotype Gallinarum strains differed in only one or two bands, supporting the view that members of these two biotypes are closely related. The same IS200 patterns were also commonly observed among strains of serotype Enteritidis, indicating its evolutionary relationship with serotype Gallinarum. NotI pulsed-field gel electrophoresis (PFGE) patterns divided strains into 24 types. Based on a similarity analysis, two clusters were formed. One contained the majority of biotype Gallinarum strains and two atypical strains of Pullorum; the other contained strains of biotype Pullorum and an otherwise typical strain of biotype Gallinarum. Two atypical strains of biotype Pullorum remained unclustered by PFGE analysis. The grouping of strains differed according to the typing method used, but the majority of strains within each of the biotypes Gallinarum and Pullorum were very similar by the chromosomal markers analysed.
采用三种基于染色体的分型方法对48株鸡伤寒沙门氏菌鸡伤寒生物型和鸡白痢生物型菌株进行了特征分析。将获得的图谱与O血清群D的其他八种沙门氏菌血清型菌株的图谱进行了比较。在两种生物型的菌株中普遍观察到相同的PvuII和PstI IS200图谱，鸡伤寒血清型菌株的三种SmaI核糖体分型仅在一两条条带上有所不同，这支持了这两种生物型的成员密切相关的观点。在肠炎血清型菌株中也普遍观察到相同的IS200图谱，表明其与鸡伤寒血清型的进化关系。NotI脉冲场凝胶电泳（PFGE）图谱将菌株分为24种类型。基于相似性分析，形成了两个簇。一个簇包含大多数鸡伤寒生物型菌株和两株非典型鸡白痢菌株；另一个簇包含鸡白痢生物型菌株和一株其他方面典型的鸡伤寒生物型菌株。两株非典型鸡白痢生物型菌株在PFGE分析中仍未聚类。菌株的分组因所用的分型方法而异，但通过分析的染色体标记，鸡伤寒生物型和鸡白痢生物型中的大多数菌株非常相似。
