Xu Lin, Li Zhi-Liang, Zhu Xiao-Xing, Ma Jun, Hong Chang-Jiang, He Jian-Xin, Xiang Ding-Cheng, Qiu Jian
Department of Cardiology, Guangzhou General Hospital of Guangzhou Military Direct, Guangzhou 510010, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Sep;25(9):819-21.
To study the expression of inducible co-stimulator ligand(ICOSL) on human coronary artery endothelial cells(HCAEC) and its being interferentialed by oxidized low density lipoprotein(ox-LDL).
ICOSL expression levels were determined by the fluorescence, reverse transcription PCR (RT-PCR) and Western blot, respectively.
The ICOSL mRNA OD values of control and 100 mg/L ox-LDL group was 0.071+/-0.035 and 0.186+/-0.044, respectively. The Western blot A values of control and 100 mg/L ox-LDL group was 10.88+/-1.53 and 16.03+/-4.08, respectively. ox-LDL increased expression of ICOSL mRNA and protein(P<0.05).
ICOSL can express on the HCAEC. ox-LDL can up-regulate the expression of ICOSL.
研究诱导性共刺激分子配体（ICOSL）在人冠状动脉内皮细胞（HCAEC）上的表达及其受氧化型低密度脂蛋白（ox-LDL）干扰的情况。
分别采用荧光法、逆转录聚合酶链反应（RT-PCR）及蛋白质免疫印迹法检测ICOSL的表达水平。
对照组及100mg/L氧化型低密度脂蛋白组ICOSL信使核糖核酸光密度值分别为0.071±0.035和0.186±0.044。对照组及100mg/L氧化型低密度脂蛋白组蛋白质免疫印迹A值分别为10.88±1.53和16.03±4.08。氧化型低密度脂蛋白增加了ICOSL信使核糖核酸及蛋白的表达（P<0.05）。
ICOSL可在人冠状动脉内皮细胞上表达。氧化型低密度脂蛋白可上调ICOSL的表达。
