Lin H J, Lai C L, Lau J Y, Chung H T, Lauder I J, Fong M W
Clinical Biochemistry Unit, University of Hong Kong.
Lancet. 1990 Jul 28;336(8709):208-12. doi: 10.1016/0140-6736(90)91735-s.
To study the heterogeneity of hepatitis B virus (HBV) DNAs in Hong Kong, where HBV infection is endemic, serum specimens from 90 HBsAg carrier children were systematically tested with nine oligonucleotide probes representing conserved sequences in the viral genome. In a pair of twins and their cousin (belonging to family H) and an unrelated child (family Y) serum HBV DNA annealed to all but one probe, a sequence located between the core and pre-S regions of the viral genome (positions 2723-2738; EcoRI site 1). Serum from the H twins' aunt and the father and paternal grandparents in the Y family were also HBV DNA-positive. The nucleotide sequences in positions 2701-2800 were analysed. The same point mutation, C to T in position 2735, was present in the HBV DNAs from 7 individuals in the two families. All 4 H family members had the same HBV DNA sequence. The HBV DNA sequences found in the Y daughter, father, and grandfather were identical and they were different from the H family mutant. These results provided evidence at the DNA level of intrafamilial transmission within these Chinese families.
为研究乙型肝炎病毒（HBV）DNA在香港的异质性（香港为HBV感染的地方性流行区），我们用代表病毒基因组保守序列的9种寡核苷酸探针，对90名HBsAg携带者儿童的血清标本进行了系统检测。在一对双胞胎及其表亲（属于H家族）和一名非亲属儿童（Y家族）中，血清HBV DNA与除一个探针外的所有探针退火，该探针对应的序列位于病毒基因组的核心区和前S区之间（位置2723 - 2738；EcoRI位点1）。H家族双胞胎的姑姑以及Y家族孩子的父亲和祖父母的血清HBV DNA也呈阳性。对位置2701 - 2800的核苷酸序列进行了分析。两个家族的7名个体的HBV DNA中存在相同的点突变，即位置2735处的C突变为T。H家族的所有4名成员具有相同的HBV DNA序列。在Y家族的女儿、父亲和祖父中发现的HBV DNA序列相同，且与H家族的突变体不同。这些结果在DNA水平上为这些中国家庭中的家族内传播提供了证据。
