Brown J M, Harken A H, Sharefkin J B
Department of Surgery, University of Colorado Health Science Center, Denver 80262.
Ann Surg. 1990 Aug;212(2):178-86. doi: 10.1097/00000658-199008000-00011.
We have the ability to isolate DNA from tissue, determine its base-pair sequence, and ask if a gene of interest is present. DNA strands can be isolated from one type of cell or organism, cleaved, and inserted (recombined) with DNA from another cell or organism. Recombinant DNA techniques have already improved health care by providing clinically useful quantities of pure human protein hormones such as erythropoietin, insulin, and growth hormone. Furthermore these techniques may increase our understanding of cellular growth control mechanisms to a level that was previously unattainable. They will also increase our knowledge of the development of major diseases and provide a means of specific nontoxic therapies for these diseases. Surgeons will need to understand basic DNA research terminology to keep up with the revolution in medical therapies that these techniques will cause. Our purpose is to begin the process of linking surgery to DNA.
我们有能力从组织中分离出DNA，确定其碱基对序列，并检测是否存在感兴趣的基因。DNA链可以从一种类型的细胞或生物体中分离出来，进行切割，然后与来自另一种细胞或生物体的DNA进行插入（重组）。重组DNA技术已经通过提供临床上有用数量的纯人类蛋白质激素，如促红细胞生成素、胰岛素和生长激素，改善了医疗保健。此外，这些技术可能会将我们对细胞生长控制机制的理解提高到一个以前无法达到的水平。它们还将增加我们对主要疾病发展的认识，并为这些疾病提供特定的无毒治疗方法。外科医生需要了解基础DNA研究术语，以跟上这些技术将引发的医学治疗革命。我们的目的是开启将外科手术与DNA联系起来的进程。
