Bertram Rebecca M, Takemoto Jody K, Remsberg Connie M, Vega-Villa Karina R, Sablani Shyam, Davies Neal M
Department of Pharmaceutical Sciences and Pharmacology, Washington State University, Pullman, 99164-6534, USA.
Biomed Chromatogr. 2010 May;24(5):516-21. doi: 10.1002/bmc.1320.
A high-performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna C(18) column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5-100.0 microg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069-18.4% (RSD%), and within 20% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <15% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and to nutraceutical product analysis.
开发了一种高效液相色谱（HPLC）法用于分析芪类化合物氧化白藜芦醇。该方法采用Luna C(18)柱，在320 nm处进行紫外检测。流动相由乙腈、水和甲酸（30:70:0.04 v/v）组成，流速为0.6 mL/min。校准曲线在0.5 - 100.0 μg/mL范围内呈线性。平均提取效率在98.9%至109%之间。该测定法的精密度为0.069 - 18.4%（相对标准偏差），在定量限（0.5 μg/mL）处相对标准偏差在20%以内。该测定法的偏差<15%，在定量限处偏差在15%以内。该测定法已成功应用于大鼠尿液的临床前药代动力学样品以及营养保健品分析。
