尿液中柚皮素的立体特异性高效液相色谱分析

Stereospecific high-performance liquid chromatographic analysis of naringenin in urine.

作者信息

Yáñez Jaime A, Davies Neal M

机构信息

College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program, Washington State University, Pullman, 99164 6534, USA.

出版信息

J Pharm Biomed Anal. 2005 Sep 1;39(1-2):164-9. doi: 10.1016/j.jpba.2005.02.025. Epub 2005 Apr 1.

DOI:10.1016/j.jpba.2005.02.025

PMID:16085136

Abstract

A method of analysis of naringenin [(+/-)-4',5,7-trihydroxyflavanone] in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism, tissue distribution in fruits and humans. A simple high-performance liquid chromatographic method was developed for simultaneous determination of naringenin enantiomers in rat and human urine. Urine (0.1 ml) was precipitated with cold acetonitrile after addition of the internal standard, daidzein. Separation was achieved on a Chiralcel OD-RH column with UV detection at 292 nm. The calibration curves were linear ranging from 0.5 to 100 microg/ml for each enantiomer. The mean extraction efficiency was >99%. Precision of the assay was <9.4% (CV), and was within 5.4% at the limit of quantitation (0.5 microg/ml). Bias of the assay was lower than 16%, and was within 15% at the limit of quantitation. The assay was applied successfully to the urinary excretion of naringenin in rats and humans.

摘要

为研究柚皮素[（±）-4′,5,7-三羟基黄烷酮]在体外和体内的代谢动力学、在水果和人体中的组织分布，需要一种分析生物流体中柚皮素的方法。已开发出一种简单的高效液相色谱法，用于同时测定大鼠和人尿液中的柚皮素对映体。加入内标黄豆苷元后，用冷乙腈沉淀尿液（0.1 ml）。在Chiralcel OD-RH柱上进行分离，于292 nm处进行紫外检测。每种对映体的校准曲线在0.5至100 μg/ml范围内呈线性。平均提取效率>99%。该测定法的精密度<9.4%（变异系数），在定量限（0.5 μg/ml）处为5.4%以内。该测定法的偏差低于16%，在定量限处为15%以内。该测定法已成功应用于大鼠和人体中柚皮素的尿排泄研究。