Mérida A, Leurentop L, Candau P, Florencio F J
Departamento de Bioquímica Vegetal y Biología Molecular, Universidad de Sevilla-CSIC, Spain.
J Bacteriol. 1990 Aug;172(8):4732-5. doi: 10.1128/jb.172.8.4732-4735.1990.
Glutamine synthetases (GSs) from two cyanobacteria, one unicellular (Synechocystis sp. strain PCC 6803) and the other filamentous (Calothrix sp. strain PCC 7601 [Fremyella diplosiphon]), were purified to homogeneity. The biosynthetic activities of both enzymes were strongly inhibited by ADP, indicating that the energy charge of the cell might regulate the GS activity. Both cyanobacteria exhibited an ammonium-mediated repression of GS synthesis. In addition, the Synechocystis sp. showed an inactivation of GS promoted by ammonium that had not been demonstrated previously in cyanobacteria.
从两种蓝细菌中纯化出谷氨酰胺合成酶（GSs），一种是单细胞的（聚球藻属PCC 6803菌株），另一种是丝状的（眉藻属PCC 7601菌株[双歧藻]），并使其达到同质状态。两种酶的生物合成活性均受到ADP的强烈抑制，这表明细胞的能量电荷可能调节GS活性。两种蓝细菌都表现出铵介导的GS合成抑制。此外，聚球藻属菌株显示出由铵促进的GS失活，这在蓝细菌中以前尚未得到证实。
