Pan Jin Bing, Hou Yu Hong, Zhang Guo Jun
Department of Respiratory Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Respirology. 2009 Sep;14(7):975-82. doi: 10.1111/j.1440-1843.2009.01606.x.
Pulmonary fibrosis has a poor prognosis. The pathogenesis of fibrotic disorders is unclear, but the extent of lung damage due to persistent inflammation is regarded as a critical factor. Rolipram inhibits inflammation induced by various stimuli, as well as the chemotaxis of fibroblasts. In this study rolipram was used to treat pulmonary fibrosis induced by bleomycin A5 in rats, and the possible mechanisms were investigated.
Rolipram (0.25 mg/kg) was administered intraperitoneally daily, following intratracheal instillation of bleomycin A5 (5 mg/kg). Animals were killed at 7 or 28 days after bleomycin A5 instillation, and indices of lung damage and fibrosis were evaluated.
Bleomycin A5 induced pulmonary inflammation and fibrosis, increased the levels of malondialdehyde and tumour necrosis factor-alpha and enhanced accumulation of collagen in the lungs. Rolipram administration significantly attenuated these effects.
Rolipram ameliorated pulmonary inflammation and fibrosis induced by bleomycin A5 in rats. The effects of rolipram may be associated with its antioxidant activity and inhibition of tumour necrosis factor-alpha production.
肺纤维化预后较差。纤维化疾病的发病机制尚不清楚，但持续炎症导致的肺损伤程度被视为关键因素。咯利普兰可抑制多种刺激诱导的炎症以及成纤维细胞的趋化作用。本研究采用咯利普兰治疗博来霉素A5诱导的大鼠肺纤维化，并探讨其可能机制。
在气管内滴注博来霉素A5（5mg/kg）后，每天腹腔注射咯利普兰（0.25mg/kg）。在博来霉素A5滴注后7天或28天处死动物，评估肺损伤和纤维化指标。
博来霉素A5诱导肺部炎症和纤维化，增加丙二醛和肿瘤坏死因子-α水平，并增强肺中胶原蛋白的积累。给予咯利普兰可显著减轻这些效应。
咯利普兰改善了博来霉素A5诱导的大鼠肺部炎症和纤维化。咯利普兰的作用可能与其抗氧化活性及抑制肿瘤坏死因子-α产生有关。
