Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway.
Arch Oral Biol. 2009 Nov;54(11):1002-7. doi: 10.1016/j.archoralbio.2009.08.005. Epub 2009 Sep 8.
In search for possible novel genes that may be involved in tooth development, we analysed the genome-wide transcriptome of developing mandibular tooth germs of mouse during embryonic and early life and selected family-with-sequence-similarity-46, member A (Fam46a) gene for further expression analysis.
We applied microarray, quantitative real time polymerase chain reaction and in situ hybridisation methods for the expression study of the mouse Fam46a gene.
We found the family-with-sequence-similarity-46, member A (Fam46a) gene to be highly expressed and further verify its temporo-spatial expression in the mouse tooth.
We have shown that Fam46a is expressed in ameloblasts' nuclei of tooth germs and hypothesise that it might act together with morphogenetic factors important for the formation of enamel in mouse tooth.
为了寻找可能参与牙齿发育的新基因，我们分析了胚胎期和生命早期下颌牙胚的全基因组转录组，并选择了 FAM46A 基因进行进一步的表达分析。
我们应用微阵列、定量实时聚合酶链反应和原位杂交方法研究了小鼠 Fam46a 基因的表达。
我们发现 FAM46A 基因在牙齿发育过程中高度表达，并进一步验证了其在小鼠牙齿中的时空表达。
我们已经表明 Fam46a 表达在牙胚的成釉细胞核中，并假设它可能与形态发生因子一起作用，这些因子对于小鼠牙齿中釉质的形成很重要。
