Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc., Naka-ku, Okayama 702-8006, Japan.
Carbohydr Res. 2009 Nov 2;344(16):2151-6. doi: 10.1016/j.carres.2009.08.016. Epub 2009 Aug 19.
The bacterial strain PP710, isolated from soil and identified as Paenibacillus species, produced a low-digestibility alpha-glucan containing a large amylase-resistant portion. This alpha-glucan was obtained in high yields from maltodextrin (dextrose equivalent 3) by using the condensed culture supernatant of the strain as the enzyme preparation. The water-soluble dietary fiber content of the low-digestibility alpha-glucan was 80.2%, and showed resistance to a rat intestinal enzyme preparation. The alpha-glucan was found to be a novel highly branched alpha-glucan by acid hydrolysis, NMR analysis, gel permeation chromatography, methylation analysis, and enzymatic digestion.
从土壤中分离到的一株名为类芽孢杆菌的细菌菌株 PP710 产生了一种低消化性的α-葡聚糖，其中含有很大一部分抗淀粉酶的部分。该α-葡聚糖可从麦芽糊精（葡萄糖当量 3）中以高产率获得，使用该菌株的浓缩培养上清液作为酶制剂。低消化性α-葡聚糖的水溶性膳食纤维含量为 80.2%，并对大鼠肠酶制剂具有抗性。通过酸水解、NMR 分析、凝胶渗透色谱、甲基化分析和酶消化发现，该α-葡聚糖是一种新型的高度支化的α-葡聚糖。
