Schmid Frank, Separovic Frances, McDougall Barbara M, Stone Bruce A, Brownlee Robert T C, Seviour Robert J
Biotechnology Research Centre, La Trobe University, Bendigo, VIC 3550, Australia.
Carbohydr Res. 2007 Nov 26;342(16):2481-3. doi: 10.1016/j.carres.2007.06.018. Epub 2007 Jun 28.
Solid state (13)C NMR studies of the extracellular glucans from the fungi Acremonium persicinum C38 (QM107a) and Acremonium sp. strain C106 indicated a backbone of (1-->3)-beta-linked glucosyl residues with single (1-->6)-beta-linked glucosyl side branches for both glucans. Analyses of enzymatic digestion products suggested that the average branching frequency for the A. persicinum glucan (66.7% branched) was much higher than that of the Acremonium sp. strain C106 glucan (28.6% branched). The solid state (13)C NMR spectra also indicated that both glucans are amorphous polymers with no crystalline regions, and the individual chains are probably arranged as triple helices.
对来自真菌嗜水枝顶孢霉C38（QM107a）和枝顶孢霉属菌株C106的胞外葡聚糖进行的固态（13）C NMR研究表明，两种葡聚糖的主链均由（1→3）-β-连接的葡萄糖基残基组成，带有单个（1→6）-β-连接的葡萄糖基侧链。酶解产物分析表明，嗜水枝顶孢霉葡聚糖的平均分支频率（66.7%分支）远高于枝顶孢霉属菌株C106葡聚糖（28.6%分支）。固态（13）C NMR光谱还表明，两种葡聚糖均为无定形聚合物，无结晶区域，且单个链可能排列为三螺旋结构。
