Riesenberg R, Nitsch C
Anatomische Anstalt, Universität München, Federal Republic of Germany.
Cell Tissue Res. 1990 Jul;261(1):107-13. doi: 10.1007/BF00329443.
The opioid peptide dynorphin A (1-17) is the third transmitter identified in the striatonigral projection, the other two being gamma-aminobutyric acid (GABA) and substance P. The ultrastructural features of the dynorphinergic terminals in substantia nigra/pars reticulata were studied using pre-embedding immunocytochemistry with the classical peroxidase-antiperoxidase-diaminobenzidine-method; these features were compared with GABAergic boutons visualized with an immunogold method. Two distinct types of dynorphin-A-immunoreactive boutons could be identified: (1) type A (81%) possessing characteristics similar to the GABAergic nerve endings in this region, i.e., large pleomorphic vesicles and symmetric synaptic contacts; (2) type B (19%) displaying asymmetric synaptic zones and small, mostly round vesicles. These results are in agreement with physiological studies suggesting a dual action of dynorphin A in substantia nigra.
阿片肽强啡肽A（1 - 17）是在纹状体黑质投射中鉴定出的第三种递质，另外两种是γ-氨基丁酸（GABA）和P物质。使用经典的过氧化物酶 - 抗过氧化物酶 - 二氨基联苯胺法进行包埋前免疫细胞化学研究黑质/网状部中强啡肽能终末的超微结构特征；将这些特征与用免疫金法可视化的GABA能终扣进行比较。可以鉴定出两种不同类型的强啡肽A免疫反应性终扣：（1）A型（81%），具有与该区域GABA能神经末梢相似的特征，即大的多形性囊泡和对称的突触接触；（2）B型（19%），显示不对称的突触区和小的、大多为圆形的囊泡。这些结果与生理学研究一致，提示强啡肽A在黑质中具有双重作用。
