Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, México.
Hum Exp Toxicol. 2009 Sep;28(9):577-82. doi: 10.1177/0960327109102798. Epub 2009 Sep 10.
The objective of the study is to determine the damage by oxidative stress induced by morphine in brain of rats fed with a protein-deficient diet. Twenty-eight malnourished male Wistar rats, 30 days old, were used in the study. The animals were divided into four groups of 7 rats per group. Group I received NaCl and the groups II; III and IV intraperitoneally received 3, 6 and 12 mg/kg of morphine sulphate, respectively, in a single dose. Animals were sacrificed and the levels of glutathione (GSH), dopamine, tryptophan and 5-hydroxyindole-3-acetic acid (5-HIAA) as well as, Na(+)/K(+) ATPase and total ATPase activity in the brain were measured. Tryptophan levels and Na(+)/K( +) ATPase activity showed non-significant changes in the experimental group. Levels of 5-HIAA decreased significantly (p = .03) in animals that received 12 mg/kg of morphine and in animals that received 3 mg/kg, levels of GSH and dopamine were found to have a significant decrease (p < .05), but a significant increase in the group that received 12 mg/kg of morphine (p < .05). Total ATPase activity increased significantly in the groups that received 3 mg/kg (p = .015) and 6 mg/kg (p = .0001) of morphine. The results show that malnutrition induces changes in cellular regulation and biochemical responses to oxidative stress caused by morphine sulphate.
本研究旨在确定营养不良饮食诱导的氧化应激对吗啡处理的大鼠脑损伤。将 28 只雄性 Wistar 幼鼠用于该研究，这些幼鼠营养不良，年龄为 30 天。将这些动物分为 4 组，每组 7 只。第 I 组接受 NaCl，第 II、III 和 IV 组分别接受 3、6 和 12mg/kg 的吗啡硫酸盐，均为单次腹腔内注射。处死动物后，测量脑内谷胱甘肽 (GSH)、多巴胺、色氨酸和 5-羟吲哚-3-乙酸 (5-HIAA)水平，以及脑内 Na(+)/K(+)ATP 酶和总 ATP 酶活性。实验组色氨酸水平和 Na(+)/K(+)ATP 酶活性无显著变化。接受 12mg/kg 吗啡的动物和接受 3mg/kg 吗啡的动物的 5-HIAA 水平显著降低 (p =.03)，接受 3mg/kg 和 12mg/kg 吗啡的动物的 GSH 和多巴胺水平显著降低 (p <.05)，但接受 12mg/kg 吗啡的动物的 GSH 和多巴胺水平显著升高 (p <.05)。接受 3mg/kg (p =.015) 和 6mg/kg (p =.0001) 吗啡的动物的总 ATP 酶活性显著增加。结果表明，营养不良会引起细胞调节变化，并对吗啡硫酸盐引起的氧化应激产生生化反应。
