Calderón Guzmán David, Barragán Mejía Gerardo, Hernández García Ernestina, Juárez Olguín Hugo
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Mexico Ciy, Mexico.
Nutr Cancer. 2006;55(2):195-200. doi: 10.1207/s15327914nc5502_11.
The aim of this study was to analyze the effect of nutritional condition and simulated exposure to ozone on Glutathione (GSH), the activity of Na+/K+ ATPase and lipid peroxidation in rat brain. Male Wistar rats were fed with 7% and 23% protein diets. Two groups were formed for each nutritional condition: one group was exposed for 15 successive days to 0.75 ppm of ozone and the other to air. Subsequently, the brain was dissected in cortex, hemispheres, cerebellum, and brainstem to measure the activity of thiobarbituric acid reactive substances (TBARS), ATPase, and levels of GSH. The activity of Na+/K+ ATPase increased in cerebellum of well-nourished rats exposed to ozone, while total ATPase and TBARS decreased in all studied areas in the malnourished groups. The levels of GSH decreased significantly (P < 0.05) in the brain of rats fed with 7% of protein diet and exposed to ozone but increased in rats fed with normal diet and exposed to ozone. These results suggest that malnutrition causes alterations in the values of Na+/K+ ATPase, total ATPase, GSH, and lipid peroxidation, while ozone contributes to these modifications. As a consequence, both variables are involved in oxidative stress in the rat brain.
本研究的目的是分析营养状况和模拟臭氧暴露对大鼠脑中谷胱甘肽（GSH）、Na+/K+ATP酶活性及脂质过氧化的影响。将雄性Wistar大鼠喂以含7%和23%蛋白质的饲料。每种营养状况下分为两组：一组连续15天暴露于0.75 ppm的臭氧中，另一组暴露于空气中。随后，将大脑解剖为皮质、半球、小脑和脑干，以测量硫代巴比妥酸反应性物质（TBARS）、ATP酶的活性以及GSH水平。暴露于臭氧的营养良好大鼠的小脑中Na+/K+ATP酶活性增加，而营养不良组所有研究区域的总ATP酶和TBARS均降低。喂食7%蛋白质饲料并暴露于臭氧的大鼠脑中GSH水平显著降低（P<0.05），而喂食正常饲料并暴露于臭氧的大鼠脑中GSH水平升高。这些结果表明，营养不良会导致Na+/K+ATP酶、总ATP酶、GSH和脂质过氧化值的改变，而臭氧会加剧这些变化。因此，这两个变量都与大鼠脑内的氧化应激有关。
