Shefner R, Mayer R, Kaushik A, D'Eustachio P, Bona C, Diamond B
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.
J Immunol. 1990 Sep 1;145(5):1609-14.
We have identified a new murine V kappa family that contains five to seven members, one member of which encodes the L chain V region of an anti-dsDNA antibody produced by a BALB/c hybridoma, C8.5. The cloned C8.5 V kappa gene exhibits highest homology with a human V kappa gene that was cloned from a nonproductive rearrangement but has never been seen in an expressed repertoire. Because this family was first identified in an autoantibody, we studied its expression in an autoimmune mouse strain. This V kappa family is expressed in 20% of hybridomas from NZB mice.
我们鉴定出了一个新的小鼠Vκ家族，该家族包含5至7个成员，其中一个成员编码由BALB/c杂交瘤C8.5产生的抗双链DNA抗体的轻链V区。克隆的C8.5 Vκ基因与一个从无生产性重排中克隆出来的人类Vκ基因具有最高的同源性，但在已表达的库中从未见过。由于这个家族最初是在自身抗体中被鉴定出来的，我们研究了它在自身免疫小鼠品系中的表达情况。这个Vκ家族在NZB小鼠20%的杂交瘤中表达。
