Ng K H, Lavigueur A, Ricard L, Boivrette M, Maclean S, Cloutier D, Gibson D M
Département de Biochimie Université de Sherbrooke, Québec, Canada.
J Immunol. 1989 Jul 15;143(2):638-48.
Germ line genes encoding mouse Ig kappa-chains belonging to the V kappa-1 group have been isolated from BALB/c, NZB, and CE, three inbred strains of differing kappa haplotype. The V kappa-1A and V kappa-1C germ line genes isolated from BALB/c (Ig kappa c) were identical to those previously described. These are the two major V kappa-1 germ line genes in BALB/c and together account for 40 of the 53 expressed V kappa-1 sequences that have been reported to date. Allelic differences in a single germ line variable region gene (V kappa-1A) in different strains of mice explain the differences in L chain IEF patterns previously associated with the Ig kappa-Ef2 locus. The rearranged kappa-gene expressed in the BALB/c myeloma MOPC-460 has been isolated and found to represent a V kappa-1A somatic variant differing by three nucleotides from the germ line V kappa-1A gene. Germ line genes isolated from NZB (Ig kappa b) and CE (Ig kappa f) show greater than 95% identity with the BALB/c genes over the 1700 nucleotides compared. Comparison by region indicated the greatest conservation of sequence occurs in and around the leader exon followed by the V-region exon. The NZB gene encodes the amino acid sequence found in the myeloma PC-2205, previously designated V kappa-1B. The V kappa-1 gene isolated from CE is likely an allele of the BALB/c V kappa-1C gene as the two share greater than 96% identity over 1700 nucleotides. The CE gene has been designated V kappa-1Cf. Ancient remnants of LINE-1 repetitive elements were detected approximately 400 bp downstream of all of the V kappa-1 genes. These possess greater homology with repetitive elements found near other kappa genes than they do with the native L1Md sequence.
已从BALB/c、NZB和CE这三种不同κ单倍型的近交系小鼠中分离出编码属于Vκ-1组的小鼠Igκ链的种系基因。从BALB/c（Igκc）分离出的Vκ-1A和Vκ-1C种系基因与先前描述的基因相同。它们是BALB/c中两个主要的Vκ-1种系基因，共同占迄今为止报道的53个表达的Vκ-1序列中的40个。不同品系小鼠中单个种系可变区基因（Vκ-1A）的等位基因差异解释了先前与Igκ-Ef2基因座相关的轻链IEF模式的差异。已分离出在BALB/c骨髓瘤MOPC-460中表达的重排κ基因，发现它代表一个Vκ-1A体细胞变体，与种系Vκ-1A基因有三个核苷酸的差异。从NZB（Igκb）和CE（Igκf）分离出的种系基因在1700个核苷酸的比较中与BALB/c基因的同一性大于95%。按区域比较表明，序列的最大保守性出现在前导外显子及其周围，其次是V区外显子。NZB基因编码在骨髓瘤PC-2205中发现的氨基酸序列，先前称为Vκ-1B。从CE分离出的Vκ-1基因可能是BALB/c Vκ-1C基因的一个等位基因，因为两者在1700个核苷酸上的同一性大于96%。CE基因已被命名为Vκ-1Cf。在所有Vκ-1基因下游约400 bp处检测到LINE-1重复元件的古老残余。与其他κ基因附近发现的重复元件相比，它们与天然L1Md序列的同源性更高。
