Prosser J S, Edwards A A, Lloyd D C
National Radiological Protection Board, Didcot, Oxon, UK.
Int J Radiat Biol. 1990 Aug;58(2):293-301. doi: 10.1080/09553009014551631.
Using aliquots from the same samples of irradiated normal human blood, T-lymphocyte survival was measured by (a) 14-day colony growth, (b) the proportion of chromosomally normal first post-irradiation metaphases and (c) the proportion of cells not suffering interphase death and delay within 48 h after irradiation. Combining (b) and (c) gives a prediction of the proportion of cytogenetically undamaged cells which reach metaphase after 48 h. These cells should be capable of producing viable colonies under suitable culture conditions. A comparison of these values with those observed in (a) shows a reasonable agreement in accordance with the hypothesis that gross chromosomal damage and cell reproductive death are closely related.
使用来自受辐照正常人血液相同样本的等分试样，通过以下方法测量T淋巴细胞存活率：(a) 14天集落生长，(b) 辐照后第一个中期染色体正常的比例，以及(c) 辐照后48小时内未发生间期死亡和延迟的细胞比例。将(b)和(c)结合起来可以预测48小时后进入中期的细胞遗传学未受损细胞的比例。这些细胞在合适的培养条件下应该能够产生活的集落。将这些值与(a)中观察到的值进行比较，结果显示出合理的一致性，这与染色体严重损伤和细胞增殖死亡密切相关的假设相符。
