Baumann M, Becker S, Krüger H J, Vogler H, Maurer T, Beck-Bornholdt H P
Institut für Biophysik und Strahlenbiologie, University of Hamburg, FRG.
Int J Radiat Biol. 1990 Aug;58(2):361-9. doi: 10.1080/09553009014551691.
A technique is presented for determining the time of metastasis during fractionated radiation therapy of the rat rhabdomyosarcoma R1H by flow cytmetric DNA index measurements. The DNA index is the relative DNA content of the tumour cells compared with normal cells. The DNA index of the highly hyperploid R1H tumour decreases linearly during radiation therapy dependent on the total dose applied, with a rate of 0.22% per Gy. Since metastatic cells escape further irradiation, their DNA index should correspond to the DNA index at time of spread. If so, cells disseminated late during a course of fractionated irradiation are expected to produce symptoms later and to have a lower DNA index compared with cells that metastasized at the start of treatment. The results obtained fitted these expectations, showing that timing of metastases by DNA flow cytometry, in principle, is possible. A first application of this method indicates that the risk per clonogenic R1H tumour cell to matastasize increases during fractionated irradiation.
本文介绍了一种通过流式细胞术测量DNA指数来确定大鼠横纹肌肉瘤R1H分次放射治疗期间转移时间的技术。DNA指数是肿瘤细胞与正常细胞相比的相对DNA含量。高度超二倍体R1H肿瘤的DNA指数在放射治疗期间根据所施加的总剂量呈线性下降，下降速率为每戈瑞0.22%。由于转移细胞逃避了进一步的照射，它们的DNA指数应与扩散时的DNA指数相对应。如果是这样，与在治疗开始时转移的细胞相比，在分次照射过程后期播散的细胞预计会在更晚的时候产生症状，并且DNA指数更低。获得的结果符合这些预期，表明原则上通过DNA流式细胞术确定转移时间是可能的。该方法的首次应用表明，在分次照射期间，每一个具有克隆形成能力的R1H肿瘤细胞发生转移的风险会增加。
