Seregi A, Simmet T, Schobert A, Hertting G
Department of Pharmacology, University of Freiburg, FRG.
J Pharm Pharmacol. 1990 Mar;42(3):191-3. doi: 10.1111/j.2042-7158.1990.tb05383.x.
The formation and composition of cysteinyl-leukotrienes (LT) in primary astroglial cell cultures prepared from newborn rat brain has been studied. Small amounts of cysteinyl-LT determined in terms of LTC4-like material in the supernatants of the cultures, became detectable after stimulation of the cells with 10(-5) M ionophore A23187. Cysteinyl-LT formation increased with time, reaching about 600 pg (mg protein)-1 after 60 min incubation. In contrast, considerable thromboxane (TX) B2 synthesis was found at 5 min following A23187-stimulation (about 30 ng TXB2 (mg protein)-1). The synthesis of cysteinyl-LT was abolished by 5 x 10(-5) M nordihydroguaiaretic acid (NDGA). Irrespective of the duration of incubation, blockage of prostanoid synthesis by 10(-6) M indomethacin did not result in increased cysteinyl-LT production. Reversed phase HPLC combined with radioimmunological detection showed that, after 60 min incubation in the presence of A23187, LTC4 and LTD4 accounted for practically all the LTC4-like immunoreactive material in the supernatants of cell cultures. No significant amounts of LTE4 could be detected. The results show that astrocytes may contribute to brain LTC4 and LTD4 synthesis. However, the cellular site of cerebral LTE4 formation seems to be other than the astroglia.
对新生大鼠脑制备的原代星形胶质细胞培养物中半胱氨酰白三烯（LT）的形成和组成进行了研究。在用10⁻⁵ M离子载体A23187刺激细胞后，培养物上清液中以LTC4样物质测定的少量半胱氨酰LT变得可检测到。半胱氨酰LT的形成随时间增加，孵育60分钟后达到约600 pg（mg蛋白质）⁻¹。相比之下，在A23187刺激后5分钟发现大量血栓素（TX）B2合成（约30 ng TXB2（mg蛋白质）⁻¹）。5×10⁻⁵ M去甲二氢愈创木酸（NDGA）消除了半胱氨酰LT的合成。无论孵育时间长短，10⁻⁶ M吲哚美辛阻断前列腺素合成均未导致半胱氨酰LT产生增加。反相高效液相色谱结合放射免疫检测显示，在A23187存在下孵育60分钟后，LTC4和LTD4几乎占细胞培养物上清液中所有LTC4样免疫反应性物质。未检测到大量LTE4。结果表明星形胶质细胞可能有助于脑LTC4和LTD4的合成。然而，脑LTE4形成的细胞部位似乎不是星形胶质细胞。
