Dores R M, Schenk L M, Meza J C
Department of Biological Sciences, University of Denver, Colorado 80208.
J Exp Zool Suppl. 1990;4:154-6. doi: 10.1002/jez.1402560429.
Acid extracts of anterior pituitaries obtained from larval, neotene, postmetamorphic young adult, and sexually active adult Ambystoma tigrinum were fractionated by gel filtration and analyzed by RIA. In larval, neotene, and postmetamorphic adults the molar ratio of ACTH(1-39) to alpha-MSH was, respectively, 1.3:1, 1:1.3, and 1:1.3. However, in sexually mature adults the molar ratio of ACTH(1-39) to alpha-MSH was 8:1. These results indicate that prior to and immediately after metamorphosis, both ACTH(1-39) and alpha-MSH are major end products of corticotropic cells in this species. As postmetamorphic animals reach sexual maturity, there is a shift in the processing of ACTH which yields ACTH(1-39) as the major end product of corticotropic cells. In the corticotropic cells of adults only trace amounts of alpha-MSH are produced.
从虎纹钝口螈的幼虫、幼态持续期个体、变态后幼年成体以及性成熟成体获取的垂体前叶酸性提取物，通过凝胶过滤进行分级分离，并采用放射免疫分析法进行分析。在幼虫、幼态持续期个体以及变态后成体中，促肾上腺皮质激素（1-39）与α-促黑素的摩尔比分别为1.3:1、1:1.3和1:1.3。然而，在性成熟成体中，促肾上腺皮质激素（1-39）与α-促黑素的摩尔比为8:1。这些结果表明，在变态之前及刚变态后，促肾上腺皮质激素（1-39）和α-促黑素都是该物种促肾上腺皮质细胞的主要终产物。随着变态后动物达到性成熟，促肾上腺皮质激素的加工过程发生转变，促肾上腺皮质激素（1-39）成为促肾上腺皮质细胞的主要终产物。在成体的促肾上腺皮质细胞中，仅产生微量的α-促黑素。
