Sumpter J P
Gen Comp Endocrinol. 1986 Jun;62(3):359-66. doi: 10.1016/0016-6480(86)90045-6.
The distribution and nature of ACTH-related material in the pituitary gland of chinook salmon (Oncorhynchus tschawytscha) was studied. Pars distalis (PD) and neurointermediate lobe (NIL) extracts were analyzed by gel filtration on BioGel P6 and the resultant fractions assayed for ACTH immunoreactivity (ACTH-IR) using six different antibodies, all of which were specific for ACTH when applied to mammalian samples, and for alpha-MSH. In the PD a number of distinct peaks of ACTH-IR were observed, which were detected to different degrees by the different ACTH antibodies. Three of the ACTH antibodies produced essentially similar results. The other ACTH antibodies produced results quite distinct from these, and distinct from one another. Thus ACTH-IR is present in the salmon PD in a number of distinct forms, all of which appear to have molecular weights between about 2000 and 5000. No alpha-MSH was detected in the PD. All of the ACTH antibodies also detected material in the NIL. In general the different antibodies produced similar results. There were fewer peaks of ACTH-IR in the NIL than in the PD. The major one of these, which eluted between the void volume and 1-39 ACTH, was detected by all the antibodies. One of the antibodies also detected a peak of ACTH-IR which eluted close to where alpha-MSH eluted. All of the ACTH antibodies detected more material in the PD than in the NIL, the ratio being about 3:1 in every case. alpha-MSH-IR eluted as a minor peak at the void volume, and a major peak in the position of authentic 1-13 alpha-MSH.
对奇努克鲑（Oncorhynchus tschawytscha）垂体中促肾上腺皮质激素（ACTH）相关物质的分布和性质进行了研究。通过在BioGel P6上进行凝胶过滤分析远侧部（PD）和神经中间叶（NIL）提取物，并使用六种不同抗体对所得级分进行ACTH免疫反应性（ACTH-IR）测定，所有这些抗体应用于哺乳动物样品时对ACTH具有特异性，对α-促黑素细胞激素（α-MSH）也具有特异性。在远侧部观察到多个不同的ACTH-IR峰，不同的ACTH抗体对其检测程度不同。三种ACTH抗体产生的结果基本相似。其他ACTH抗体产生的结果与这些结果截然不同，且彼此不同。因此，ACTH-IR以多种不同形式存在于鲑鱼远侧部中，所有这些形式的分子量似乎都在约2000至5000之间。在远侧部未检测到α-MSH。所有ACTH抗体也在神经中间叶中检测到物质。一般来说，不同抗体产生的结果相似。神经中间叶中的ACTH-IR峰比远侧部中的少。其中主要的一个峰在空体积和1-39 ACTH之间洗脱，所有抗体都能检测到。其中一种抗体还检测到一个ACTH-IR峰，其洗脱位置接近α-MSH的洗脱位置。所有ACTH抗体在远侧部中检测到的物质比在神经中间叶中更多，每种情况下的比例约为3:1。α-MSH-IR在空体积处作为一个小峰洗脱，在 authentic 1-13 α-MSH的位置作为一个主峰洗脱。
