Dafale Nishant, Agrawal Leena, Kapley Atya, Meshram Sudhir, Purohit Hemant, Wate Satish
Environmental Impact & Risk Assessment Division and Environmental Genomic Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, India.
Bioresour Technol. 2010 Jan;101(2):476-84. doi: 10.1016/j.biortech.2009.08.006. Epub 2009 Sep 11.
Dye degradation has gained attention of late due to indiscriminate disposal from user industries. Enhancing efficiency of biological treatment provides a cheaper alternative vis-à-vis other advanced technologies. Dye molecules are metabolized biologically via anoxic and oxic treatments. In this study, bacterial community surviving on dye effluent working in anoxic-oxic bioreactor was analyzed using 16S rDNA approach. Azo-dye decolorizing and degrading bacterial community was enriched in lab-scale two-stage anoxic-oxic bioreactor. 16S rDNA metagenomic libraries of enriched population were constructed, screened and phylogenetically analyzed separately. Removal of approximately 35% COD with complete decolorization was observed in anoxic bioreactor. Process was carried out by uncultured gamma proteobacterium constituting 48% of the total population and 12% clones having homology to Klebsiella. Aromatic amines generated during partial treatment under anoxic bioreactor were treated by aerobic population having 72% unculturable unidentified bacterium and rest of the population consisting of Thauera sp., Pseudoxanthomonas sp., Desulfomicrobium sp., Ottowia sp., Acidovorax sp., and Bacteriodetes bacterium sp.
近年来，由于用户行业的随意排放，染料降解受到了关注。与其他先进技术相比，提高生物处理效率提供了一种更便宜的选择。染料分子通过缺氧和有氧处理进行生物代谢。在本研究中，使用16S rDNA方法分析了在缺氧-好氧生物反应器中处理染料废水的细菌群落。在实验室规模的两级缺氧-好氧生物反应器中富集了偶氮染料脱色和降解细菌群落。分别构建、筛选和系统发育分析了富集群体的16S rDNA宏基因组文库。在缺氧生物反应器中观察到约35%的化学需氧量（COD）被去除且完全脱色。该过程由未培养的γ-变形菌完成，其占总群体的48%，12%的克隆与克雷伯氏菌具有同源性。在缺氧生物反应器中部分处理过程中产生的芳香胺由需氧群体处理，其中72%为不可培养的未鉴定细菌，其余群体包括陶厄氏菌属、假黄单胞菌属、脱硫微菌属、奥托氏菌属、嗜酸菌属和拟杆菌属细菌。
