Life Science College, Northeast Forestry University, Harbin, 150040, China.
J Hazard Mater. 2012 Jun 30;221-222:185-92. doi: 10.1016/j.jhazmat.2012.04.032. Epub 2012 Apr 20.
Thirteen pure strains that possessed high methyl red (MR)-decolorizing ability were isolated from dye-contaminated water. Each isolate was identified by 16S rDNA sequencing. The results reveal that all of the isolated strains were facultative anaerobic bacteria. Two novel bacterial consortia (AE and AN), which could decolorize MR under aerobic and anaerobic conditions, respectively, were developed. Azo dye decolorization rate was significantly higher with the use of consortia compared to that with the use of individual strains. Both of the consortia can decolorize different azo dyes effectively in a short time, and tolerate MR with high concentrations. To provide further insight into the microbial diversity of the bacteria consortia under aerobic and anaerobic conditions, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses were performed. PCR-DGGE profiles revealed that the microbial community had changed significantly with varying initial concentrations of MR. Phylogenetic analysis indicated that microbial populations in the aerobic compartment belong to Klebsiella, Buttiauxella and Bacillus, whereas Klebsiella, Escherichia, Bacillus and Clostridium were present in the anaerobic compartment. Klebsiella, which was the majority genus in both of the consortia, may play an important role in azo dye removal.
从染料污染水中分离到 13 株具有较高甲基红（MR）脱色能力的纯菌株。通过 16S rDNA 测序对每个分离株进行鉴定。结果表明，所有分离株均为兼性厌氧菌。分别开发了两种新型细菌共生体（AE 和 AN），它们可以在有氧和厌氧条件下分别脱色 MR。与单独使用菌株相比，使用共生体可以显著提高偶氮染料的脱色率。这两种共生体都可以在短时间内有效脱色不同的偶氮染料，并且可以耐受高浓度的 MR。为了进一步了解有氧和厌氧条件下细菌共生体的微生物多样性，进行了聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析。PCR-DGGE 图谱表明，随着初始 MR 浓度的变化，微生物群落发生了显著变化。系统发育分析表明，好氧隔室中的微生物种群属于克雷伯氏菌属、布氏杆菌属和芽孢杆菌属，而厌氧隔室中存在克雷伯氏菌属、大肠杆菌属、芽孢杆菌属和梭菌属。在两种共生体中均占优势的克雷伯氏菌可能在偶氮染料去除中发挥重要作用。
