Department of Chemistry, University of Tennessee, Knoxville, Tennessee, USA.
J Am Soc Mass Spectrom. 2009 Nov;20(11):1983-7. doi: 10.1016/j.jasms.2009.07.022. Epub 2009 Aug 12.
Using alternative enzymes for on-line digestion with a triaxial electrospray probe extends sequence coverage. This is the first report of utilization of our triaxial probe for on-line analysis with enzymes other than pepsin, suggesting potential for broader application. The probe allows access to processes occurring on a timescale and/or involving substrate conformations complementary to those for conventional (off-line) digestion. Some of the features observed in application to Abeta fibrils are suggestive of unique reactive intermediates during dissolution. Data obtained with enzyme mixtures suggest synergistic effects.
使用替代酶与三轴电喷雾探头进行在线消化可扩展序列覆盖范围。这是首次报道使用我们的三轴探头与胃蛋白酶以外的酶进行在线分析，表明具有更广泛的应用潜力。该探头允许访问在时间尺度和/或涉及与传统（离线）消化互补的底物构象上发生的过程。在应用于 Abeta 纤维时观察到的一些特征表明在溶解过程中存在独特的反应中间体。用酶混合物获得的数据表明存在协同效应。
