Tachibana Keisuke, Takeuchi Kentaro, Inada Hirohiko, Yamasaki Daisuke, Ishimoto Kenji, Tanaka Toshiya, Hamakubo Takao, Sakai Juro, Kodama Tatsuhiko, Doi Takefumi
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
Biochem Biophys Res Commun. 2009 Nov 20;389(3):501-5. doi: 10.1016/j.bbrc.2009.09.018. Epub 2009 Sep 11.
Solute carrier family 25, member 20 (SLC25A20) is a key molecule that transfers acylcarnitine esters in exchange for free carnitine across the mitochondrial membrane in the mitochondrial beta-oxidation. The peroxisome proliferator-activated receptor alpha (PPARalpha) is a ligand-activated transcription factor that plays an important role in the regulation of beta-oxidation. We previously established tetracycline-regulated human cell line that can be induced to express PPARalpha and found that PPARalpha induces the SLC25A20 expression. In this study, we analyzed the promoter region of the human slc25a20 gene and showed that PPARalpha regulates the expression of human SLC25A20 via the peroxisome proliferator responsive element.
溶质载体家族25成员20（SLC25A20）是一种关键分子，在线粒体β氧化过程中，它能跨线粒体膜转运酰基肉碱酯以交换游离肉碱。过氧化物酶体增殖物激活受体α（PPARα）是一种配体激活的转录因子，在β氧化的调节中起重要作用。我们之前建立了可诱导表达PPARα的四环素调控人细胞系，并发现PPARα可诱导SLC25A20表达。在本研究中，我们分析了人slc25a20基因的启动子区域，并表明PPARα通过过氧化物酶体增殖物反应元件调节人SLC25A20的表达。
