Research Institute for the Biology of Farm Animals (FBN) Dummerstorf, Dummerstorf, Germany.
Mol Cell Probes. 2010 Feb;24(1):32-7. doi: 10.1016/j.mcp.2009.09.001. Epub 2009 Sep 11.
ZDHHC9 (zinc finger, DHHC-type containing 9) is a protein acyl transferase involved in palmitoylation of basic signaling molecules. We found ZDHHC9 expression increased in hind leg muscles of newborn splay leg piglets. In order to elucidate the background of this increased expression we determined the structure of the porcine gene, including sequence variation, and analyzed the structure and expression of microRNAs potentially targeting the gene. We confirmed the expression results by RT Real-time PCR. The porcine ZDHHC9 gene has a similar structure to the human gene with two transcripts resulting in an identical protein. None of the 17 single nucleotide polymorphisms (SNPs) identified in the porcine gene affects the protein or putative microRNA binding sites, respectively. Two microRNAs (93 [minor] and 106b) were assayed in the muscles. Their expression variation proved to be independent from ZDHHC9 expression thus eliminating them as causally related to congenital splay leg.
ZDHHC9（锌指 DHHC 型含 9 个）是一种参与碱性信号分子棕榈酰化的蛋白酰基转移酶。我们发现，ZDHHC9 在先天性八字腿仔猪后腿肌肉中的表达增加。为了阐明这种表达增加的背景，我们确定了猪基因的结构，包括序列变异，并分析了可能针对该基因的 microRNA 的结构和表达。我们通过 RT Real-time PCR 证实了表达结果。猪 ZDHHC9 基因的结构与人基因相似，产生两种转录本，导致产生相同的蛋白质。在猪基因中鉴定的 17 个单核苷酸多态性（SNP）中，没有一个分别影响蛋白质或假定的 microRNA 结合位点。在肌肉中检测到两种 microRNAs（93[次要]和 106b）。它们的表达变化与 ZDHHC9 表达无关，因此排除了它们与先天性八字腿的因果关系。
