Shakleya Diaa M, Huestis Marilyn A
Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Nov 1;877(29):3537-42. doi: 10.1016/j.jchromb.2009.08.033. Epub 2009 Aug 28.
An LC-MS/MS method for the simultaneous quantification of nicotine, cotinine, trans-3'-hydroxycotinine and norcotinine in human plasma was developed and fully validated. Potential endogenous and exogenous interferences were extensively evaluated and limits of quantification were determined by decreasing analyte concentration. Analytical ranges were 1-500 ng/mL for nicotine and cotinine, 5-500 ng/mL for trans-3'-hydroxycotinine and norcotinine. Mean intra- and inter-assay analytical recoveries were between 101.9 and 116.8%, and intra- and inter-assay imprecision were less than 11% RSD for all analytes: parameters were evaluated at three different concentrations across the linear range of the assay. Extraction efficiency was > or = 70% for all analytes. This validated method is useful for the determination of nicotine and metabolites in human plasma to support research on the role of nicotine biomarkers on neuronal systems mediating cognitive and affective processes and to differentiate active, passive and environmental exposure.
建立了一种用于同时定量测定人血浆中尼古丁、可替宁、反式-3'-羟基可替宁和去甲可替宁的液相色谱-串联质谱法，并进行了全面验证。广泛评估了潜在的内源性和外源性干扰，并通过降低分析物浓度确定了定量限。尼古丁和可替宁的分析范围为1-500 ng/mL，反式-3'-羟基可替宁和去甲可替宁的分析范围为5-500 ng/mL。所有分析物的平均批内和批间分析回收率在101.9%至116.8%之间，批内和批间不精密度小于11%RSD：在整个分析线性范围内的三个不同浓度下评估了这些参数。所有分析物的提取效率≥70%。这种经过验证的方法可用于测定人血浆中的尼古丁及其代谢物，以支持关于尼古丁生物标志物在介导认知和情感过程的神经系统中的作用的研究，并区分主动、被动和环境暴露。
