Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Department of Bio and Environmental Technology, Division of Environmental and Life Science, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1783-1787. doi: 10.1099/ijs.0.018358-0. Epub 2009 Sep 11.
Two novel strains of Gram-negative, non-sporulating, short rod-shaped, motile bacteria, designated DCY25T and DCY28, were isolated from soil of a ginseng field in South Korea and characterized in order to determine their taxonomic position. 16S rRNA gene sequence analysis showed that strains DCY25T and DCY28 belonged to the Betaproteobacteria, the highest sequence similarities being found with Pusillimonas noertemannii BN9T (96.9%), Bordetella trematum DSM 11334(T) (95.9%), Achromobacter denitrificans DSM 30026T (95.9%), Achromobacter insolitus LMG 6003T (95.8%) and Pigmentiphaga kullae K24T (95.5%). Chemotaxonomic data revealed that both strains DCY25T and DCY28 possessed ubiquinone Q-8. Fatty acid analysis of strain DCY25T demonstrated the presence of 19:0 cyclo omega8c (22.8%) and 16:0 (16.6%). The polar lipid profiles of strains DCY25T and DCY28 included phosphatidylethanolamine, phosphatidylglycerol, two unknown aminolipids and diphosphatidylglycerol. The G+C contents of strains DCY25T and DCY28 were 57.3 and 57.2 mol%, respectively. DNA-DNA relatedness values, biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strains DCY25T and DCY28 from Pusillimonas noertemannii. Therefore, strains DCY25T and DCY28 should be classified in a novel species, for which the name Pusillimonas ginsengisoli sp. nov. is proposed. The type strain is DCY25T (=KCTC 22046T =JCM 14767T); strain DCY28 (=KCTC 22047=JCM 14768) is a reference strain.
两株新型革兰氏阴性、非孢子形成、短杆状、能动细菌，分别命名为 DCY25T 和 DCY28，从韩国人参田土壤中分离出来，并对其进行了特征描述，以确定其分类地位。16S rRNA 基因序列分析表明，菌株 DCY25T 和 DCY28 属于β变形菌门，与 Pusillimonas noertemannii BN9T（96.9%）、Bordetella trematum DSM 11334（T）（95.9%）、Achromobacter denitrificans DSM 30026T（95.9%）、Achromobacter insolitus LMG 6003T（95.8%）和 Pigmentiphaga kullae K24T（95.5%）的相似度最高。化学分类数据表明，两株菌株 DCY25T 和 DCY28 都含有泛醌 Q-8。菌株 DCY25T 的脂肪酸分析表明存在 19:0 环 ω8c（22.8%）和 16:0（16.6%）。菌株 DCY25T 和 DCY28 的极性脂谱包括磷脂酰乙醇胺、磷脂酰甘油、两种未知的氨基脂和双磷脂酰甘油。菌株 DCY25T 和 DCY28 的 G+C 含量分别为 57.3%和 57.2%mol%。菌株 DCY25T 和 DCY28 的 DNA-DNA 同源性值、生化和生理特征强烈支持它们在基因型和表型上与 Pusillimonas noertemannii 的分化。因此，菌株 DCY25T 和 DCY28 应归类于一个新种，命名为人参短小假单胞菌（Pusillimonas ginsengisoli）。模式菌株为 DCY25T（=KCTC 22046T=JCM 14767T）；菌株 DCY28（=KCTC 22047=JCM 14768）为参考菌株。
