Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2326-2330. doi: 10.1099/ijs.0.020404-0. Epub 2009 Nov 20.
A Gram-negative, motile, non-spore-forming bacterial strain, designated MJ07(T), was isolated from a farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ07(T) belongs to the family Alcaligenaceae, class Betaproteobacteria, and is related most closely to Pusillimonas ginsengisoli KCTC 22046(T) (98.6 % sequence similarity) and Pusillimonas noertemannii BN9(T) (96.9 %). The levels of 16S rRNA gene sequence similarity between strain MJ07(T) and members of all other recognized species of the family Alcaligenaceae were below 95.2 %. The G+C content of the genomic DNA of strain MJ07(T) was 59.4 mol%. The detection of a quinone system with ubiquinone Q-8 as the major respiratory lipoquinone, putrescine as the predominant polyamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unknown aminolipids as major polar lipids and a fatty acid profile with C₁₆:₀ (32.0 %), C₁₇:₀cyclo (24.7 %) and C₁₉:₀cyclo ω8c (11.5 %) as the major components supported the affiliation of strain MJ07(T) to the genus Pusillimonas. Strain MJ07(T) exhibited relatively low levels of DNA-DNA relatedness with respect to P. ginsengisoli KCTC 22046(T) (50 ± 8 %) and P. noertemannii KACC 13183(T) (18 ± 7 %). On the basis of its phenotypic and genotypic properties together with its phylogenetic distinctiveness, strain MJ07(T) (=KCTC 22455(T) =JCM 16386(T)) should be classified in the genus Pusillimonas as the type strain of a novel species, for which the name Pusillimonas soli sp. nov. is proposed.
一株革兰氏阴性、运动、非孢子形成的细菌菌株，被命名为 MJ07(T)，从农田土壤中分离出来，通过多相研究来确定其分类地位。比较 16S rRNA 基因序列分析表明，菌株 MJ07(T)属于黄杆菌科，β变形菌纲，与 P. ginsengisoli KCTC 22046(T)（98.6%序列相似性）和 P. noertemannii BN9(T)（96.9%）最为密切相关。菌株 MJ07(T)与黄杆菌科所有其他公认种的 16S rRNA 基因序列相似性水平均低于 95.2%。菌株 MJ07(T)的基因组 DNA G+C 含量为 59.4mol%。检测到醌类系统，以泛醌 Q-8 作为主要呼吸类脂醌，腐胺作为主要聚胺，磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和两种未知的氨基脂类作为主要极性脂类，脂肪酸谱以 C16:0（32.0%）、C17:0cyclo（24.7%）和 C19:0cyclo ω8c（11.5%）为主要成分，支持菌株 MJ07(T)属于 Pusillimonas 属。与 P. ginsengisoli KCTC 22046(T)（50±8%）和 P. noertemannii KACC 13183(T)（18±7%）相比，菌株 MJ07(T)的 DNA-DNA 同源性水平相对较低。基于其表型和基因型特性以及系统发育上的独特性，菌株 MJ07(T)（=KCTC 22455(T)=JCM 16386(T)）应归类于 Pusillimonas 属，为一个新种的模式菌株，命名为土壤寡养单胞菌（Pusillimonas soli）。
