RNA诱导沉默复合体（RISC）组装与功能的生化剖析

Biochemical dissection of RISC assembly and function.

作者信息

Tomari Yukihide

机构信息

Department of Medical Genome Sciences, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2009(53):15. doi: 10.1093/nass/nrp008.

DOI:10.1093/nass/nrp008

PMID:19749237

Abstract

Small silencing RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs), regulate expression of their target genes via RNA-induced silencing complex (RISC). RISC assembly follows complex, ordered pathways, and RISC function is as diverse as cleavage, translational repression, and deadenylation of the target. We have recently shown how siRNAs and miRNAs are assembled into distinct types of RISC, and how differently they function, using Drosophila as a model organism.

摘要

小干扰RNA，包括小干扰RNA（siRNA）和微小RNA（miRNA），通过RNA诱导沉默复合体（RISC）调控其靶基因的表达。RISC组装遵循复杂且有序的途径，并且RISC的功能多样，包括对靶标的切割、翻译抑制和去腺苷酸化。我们最近利用果蝇作为模式生物，展示了siRNA和miRNA如何组装成不同类型的RISC，以及它们功能上的差异。