Abe Hiroshi, Abe Naoko, Uda Miwako, Tsuneda Satoshi, Ito Yoshihiro
RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
Nucleic Acids Symp Ser (Oxf). 2009(53):65-6. doi: 10.1093/nass/nrp033.
We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.
我们设计并合成了用于RNA干扰应用的哑铃状纳米环状RNA，其由一个茎和两个环组成(1)。RNA哑铃被人类Dicer酶特异性识别并切割，因此在细胞中转化为双链RNA，尽管这种RNA在血清中抗降解。对该结构进行了优化，以使其RNAi活性最大化。当茎长度为23个碱基对时，可实现最强活性。与正常小干扰RNA相比，该分子的形状（一种无末端结构）延长了RNAi活性。
