芽殖酵母端粒DNA的四重结构

Tetraplex structure of budding yeast telomeric DNA.

作者信息

Torigoe Hidetaka, Horio Eugene, Takehara Takashi, Kaneda Kaoru, Kozasa Tetsuo

机构信息

Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, Tokyo 162-8601, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2009(53):235-6. doi: 10.1093/nass/nrp118.

DOI:10.1093/nass/nrp118

PMID:19749347

Abstract

We examined the structural properties of budding yeast telomeric DNA sequences, SCTELG4: 5'-(TGGG TGTG)(4)-3' and SCTELGG4: 5'-(TGGGTGTGG)(4)-3', in the presence of Na(+) or K(+). The conformation of SCTELG4 with Na(+) was a mixture of parallel tetraplex DNA and unstructured single-stranded DNA. On the other hand, the conformation of SCTELGG4 with Na(+) was a mixture of parallel and antiparallel tetraplex DNA. The ratio of the amount of parallel tetraplex to that of unstructured single-strand was increased for SCTELG4 by the presence of K(+). The conformation of almost all of SCTELGG4 was changed into parallel tetraplex by the presence of K(+). These results indicate that structural change of SCTELG4 and SCTELGG4 may be induced by the type of cation. We conclude that the conformation of budding yeast telomeric DNA sequences depends on the base sequence and the type of cation.

摘要

我们研究了在存在Na⁺或K⁺的情况下，出芽酵母端粒DNA序列SCTELG4: 5'-(TGGG TGTG)(4)-3'和SCTELGG4: 5'-(TGGGTGTGG)(4)-3'的结构特性。含有Na⁺时，SCTELG4的构象是平行四链体DNA和无结构单链DNA的混合物。另一方面，含有Na⁺时，SCTELGG4的构象是平行和反平行四链体DNA的混合物。K⁺的存在使SCTELG4中平行四链体与无结构单链的量之比增加。K⁺的存在使几乎所有SCTELGG4的构象都转变为平行四链体。这些结果表明，SCTELG4和SCTELGG4的结构变化可能由阳离子类型诱导。我们得出结论，出芽酵母端粒DNA序列的构象取决于碱基序列和阳离子类型。