小鼠端粒DNA结合蛋白Pot1与小鼠端粒DNA相互作用导致小鼠端粒DNA四链体结构解折叠。

Unfolding of tetraplex structure of mouse telomeric DNA by the interaction with mouse telomeric DNA binding protein Pot1.

作者信息

Torigoe Hidetaka, Kaneda Kaoru

机构信息

Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2007(51):249-50. doi: 10.1093/nass/nrm125.

DOI:10.1093/nass/nrm125

PMID:18029680

Abstract

We analyzed the structural properties of mouse telomeric DNA sequence, Tel3.5: 5'-AGGG(TTAGG G)3-3', and nontelomeric DNA sequence, T22: 5'-T22-3', and examined the interaction with a single-stranded telomeric DNA-binding domain of mouse telomeric DNA-binding protein Pot1 (mPot1DBD). T22 did not form any higher-order structure, but Tel3.5 formed antiparallel tetraplex structure in the presence of Na(+). The antiparallel tetraplex of Tel3.5 became unfolded upon the interaction with mPot1DBD. Considering that the antiparallel tetraplex is known to inhibit telomerase-mediated telomere elongation, we conclude that the ability of Pot1 to unfold the antiparallel tetraplex of the telomeric DNA is required for regulation of telomerase-mediated telomere elongation.

摘要

我们分析了小鼠端粒DNA序列Tel3.5（5'-AGGG(TTAGGG)3-3'）和非端粒DNA序列T22（5'-T22-3'）的结构特性，并研究了它们与小鼠端粒DNA结合蛋白Pot1的单链端粒DNA结合结构域（mPot1DBD）的相互作用。T22未形成任何高阶结构，但Tel3.5在存在Na(+)的情况下形成了反平行四链体结构。Tel3.5的反平行四链体在与mPot1DBD相互作用时会展开。鉴于已知反平行四链体会抑制端粒酶介导的端粒延长，我们得出结论，Pot1展开端粒DNA反平行四链体的能力是调节端粒酶介导的端粒延长所必需的。