5'-末端封闭结构在真核生物蛋白质合成中对稳定mRNA的重要性。
Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.
作者信息
Shimotohno K, Kodama Y, Hashimoto J, Miura K I
出版信息
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2734-8. doi: 10.1073/pnas.74.7.2734.
Abstract
The 7-methylguanylic acid residue confronting the 5'-terminal nucleotide of mRNA through two pyrophosphate linkages was completely removed by tobacco pyrophosphatase from mRNAs of cytoplasmic polyhedrosis virus, tobacco mosaic virus (viral RNA), and globin without any scission in the inner part of the RNA chain. Protein synthesis ability in a wheat germ cell-free system was lost after this treatment of all three kinds of mRNA. The initiation complexes for protein synthesis of these three RNAs were not obtained after using tobacco phosphodiesterase-treated mRNA. On incubation of mRNA in a wheat germ extract, the mRNA lacking m7G was quickly degraded from the 5' terminus in an exonucleolytic way, whereas the intact mRNA remained stable. These results show that one of the confronting nucleotide structure's functions is to stabilize the mRNA, to prevent its degradation.
摘要
通过两个焦磷酸键与mRNA的5'-末端核苷酸相对的7-甲基鸟苷酸残基，被烟草焦磷酸酶从细胞质多角体病毒、烟草花叶病毒（病毒RNA）和珠蛋白的mRNA中完全去除，而RNA链内部没有任何断裂。在对这三种mRNA进行这种处理后，小麦胚无细胞系统中的蛋白质合成能力丧失。在用烟草磷酸二酯酶处理过的mRNA之后，无法获得这三种RNA的蛋白质合成起始复合物。将mRNA在小麦胚提取物中孵育时，缺乏m7G的mRNA会以核酸外切的方式从5'末端迅速降解，而完整的mRNA则保持稳定。这些结果表明，相对的核苷酸结构的功能之一是稳定mRNA，防止其降解。
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